Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective paper-enhanced infrared spectroscopy for molecular detection of deoxynivalenol in wheat.

Food chemistry·2026
Same author

One-pot expression of an oligoclonal nanobody-based antivenom in growth-decoupled Escherichia coli.

Trends in biotechnology·2026
Same author

Controlled immobilization of single-domain antibodies using cellulose-binding modules for AB<sub>5</sub>-type toxin detection in paper-based assays.

RSC advances·2026
Same author

Large animal models for the assessment of snakebite envenoming therapies.

npj drug discovery·2026
Same author

Generalizable Direct Protein Sequencing With InstaNexus.

Molecular & cellular proteomics : MCP·2026
Same author

An mRNA-delivered consensus allergen induces a neutralizing IgG response against food and pollen allergens.

Nature communications·2026

Related Experiment Video

Updated: Jul 4, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

5.0K

Multiplex lateral flow assay development for snake venom detection in biological matrices.

Cecilie Knudsen1,2, Selma B Belfakir3,4, Pelle Degnegaard5

  • 1VenomAid Diagnostics, 2800, Kongens Lyngby, Denmark. ckn@venomaid.com.

Scientific Reports
|January 31, 2024
PubMed
Summary

A new rapid diagnostic test can differentiate between Bothrops and Lachesis snake venoms in under 15 minutes. This monoclonal antibody-based assay aids clinicians in diagnosing snakebites, improving patient outcomes in Brazil.

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.3K
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

8.0K

Related Experiment Videos

Last Updated: Jul 4, 2025

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
07:08

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

5.0K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.3K
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

8.0K

Area of Science:

  • Medical Diagnostics
  • Immunology
  • Toxicology

Background:

  • Bothrops and Lachesis snakes are medically significant in Brazil, causing numerous envenomings annually.
  • Accurate species identification is crucial for effective antivenom treatment, but current diagnostic methods rely on syndromic approaches that can be challenging for medical staff.
  • Limited training in clinical snakebite management can hinder accurate diagnosis.

Purpose of the Study:

  • To develop a rapid, accurate diagnostic tool for differentiating Bothrops and Lachesis snake venoms.
  • To create a point-of-care test to aid medical personnel in diagnosing snakebite envenomings.
  • To improve patient outcomes by enabling timely and appropriate treatment.

Main Methods:

  • Development of a monoclonal antibody-based multiplex lateral flow assay (LFA).
  • Validation of the LFA for differentiating Bothrops and Lachesis venoms within 15 minutes.
  • Assessment of the LFA's performance in detecting venoms in spiked plasma, urine, and sera using naked-eye and smartphone-based readings.

Main Results:

  • The LFA successfully differentiates between Bothrops and Lachesis venoms in under 15 minutes.
  • The assay can detect venoms in plasma and urine across six orders of magnitude, with detection limits of 10-50 ng/mL.
  • Sensitivity in spiked sera ranged from 50-500 ng/mL for B. atrox and L. muta venoms.

Conclusions:

  • The developed LFA is a promising tool for point-of-care diagnosis of Bothrops and Lachesis snakebites in Brazil.
  • This rapid diagnostic test has the potential to support medical personnel, improve patient management, and save lives.
  • The assay's ability to detect venom in various biological matrices enhances its clinical utility.