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MiR-130a-3p inhibits endothelial inflammation by regulating the expression of MAPK8 in endothelial cells

Mingming Gu1, Kun Liu1, Hui Xiong1

  • 1Department of Cardiothoracic Surgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu Province, China.

Heliyon
|February 1, 2024
PubMed

Insights

MicroRNA-130a-3p (miR-130a-3p) protects against atherosclerosis by promoting endothelial cell proliferation and reducing inflammation. Upregulating miR-130a-3p counteracts the effects of oxidized LDL, suggesting it as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Cell Biology

Background:

  • Atherosclerosis (AS) is a chronic inflammatory disease.
  • Endothelial cell dysfunction is a key factor in AS pathogenesis.
  • MicroRNAs play crucial roles in regulating cellular processes relevant to AS.

Purpose of the Study:

  • To investigate the role of microRNA-130a-3p (miR-130a-3p) in endothelial cells (ECs) exposed to oxidized low-density lipoprotein (ox-LDL).
  • To explore the underlying molecular mechanisms of miR-130a-3p in ox-LDL-treated ECs.
  • To evaluate the therapeutic potential of miR-130a-3p in AS.

Main Methods:

  • Human umbilical vein endothelial cells (HUVECs) were treated with ox-LDL.
  • Cell proliferation and apoptosis were assessed using CCK-8, EdU, and flow cytometry.
  • Cytokine expression and protein levels were measured by ELISA and Western blot.
  • Bioinformatics and dual-luciferase reporter assays identified MAPK8 as a direct target of miR-130a-3p.

Main Results:

  • miR-130a-3p was downregulated in ox-LDL-treated HUVECs.
  • Upregulation of miR-130a-3p promoted HUVEC proliferation and inhibited apoptosis.
  • miR-130a-3p suppressed inflammatory markers (TNF-α, IL-6) and adhesion molecules (VCAM-1, ICAM-1, E-selectin).
  • MAPK8 was upregulated by ox-LDL; its silencing mimicked miR-130a-3p's protective effects.

Conclusions:

  • miR-130a-3p exerts protective effects on endothelial cells against ox-LDL-induced injury.
  • miR-130a-3p targets MAPK8, mediating its anti-inflammatory and anti-apoptotic functions.
  • miR-130a-3p represents a promising therapeutic target for atherosclerosis treatment.
Keywords:
AtherosclerosisEndothelial cellsMAPK8MiR-130a-3p

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