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Multiplex Aptamer-Based Fluorescence Assay Using Magnetism-Encoded Nanoparticles for Simultaneous Detection of
Sixuan Wu1, Yingjie Huang1, Jin Wen1
1Key Laboratory of Chemical Biology of Jiangxi Province, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, P. R. China.
Analytical Chemistry
|February 1, 2024
Summary
This study introduces a novel multiplex aptamer-based fluorescence assay for detecting multiple bacteria simultaneously. The innovative magnetism-encoded nanoparticles offer improved accuracy and simplicity for pathogen detection in complex samples.
Area of Science:
- Biotechnology
- Nanotechnology
- Analytical Chemistry
Background:
- Multiplex assays enable simultaneous detection of multiple analytes but often face accuracy and complexity issues.
- Existing methods for detecting multiple pathogenic bacteria can be cumbersome and prone to errors.
Purpose of the Study:
- To develop a simplified and accurate multiplex aptamer-based fluorescence assay for simultaneous detection of multiple pathogenic bacteria.
- To overcome the limitations of conventional multiplex assays in terms of accuracy and complexity.
Main Methods:
- Fabrication of magnetism-encoded Fe3O4@ZIF-90 (FZ) composites with distinct magnetic strengths.
- Functionalization of FZ composites with specific aptamers for sequential separation of target bacteria.
- Utilizing a single-color fluorescence label for simultaneous quantitative analysis, minimizing spectral crosstalk.
Main Results:
- Demonstrated successful sequential separation of multiple bacteria using FZ composites with varying magnetic properties.
- Achieved simultaneous quantitative analysis of multiple bacteria with high accuracy and minimal spectral interference.
- Validated the assay's applicability in complex biological matrices, such as contaminated milk samples.
Conclusions:
- The developed multiplex aptamer-based fluorescence assay offers a simple, efficient, and accurate method for simultaneous detection of multiple pathogenic bacteria.
- This approach provides valuable insights for advancing multiplex assay development and bacterial detection methodologies.
- The assay shows significant potential for application in food safety and clinical diagnostics.
