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Updated: Jul 4, 2025

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology

Safety Precautions and Operating Procedures in an ABSL-4 Laboratory: 3. Aerobiology

Published on: October 3, 2016

Operator-derived particles and falling bacteria in biosafety cabinets.

Mitsuru Mizuno¹, Koki Abe², Takashi Kakimoto³

¹Center for Stem Cell and Regenerative Medicine, Tokyo Medical and Dental University (TMDU), 1-5-45, Bunkyo-ku, Yushima, Tokyo 113-8519, Japan.

Regenerative Therapy

|February 2, 2024

Summary

Operator clothing significantly impacts particle and bacteria contamination in clean rooms. Dustless clothing drastically reduces airborne contaminants compared to textile clothing, crucial for aseptic manufacturing.

Keywords:

Biosafety cabinet Cell-product processing Colony-forming unit Falling bacteria Particle

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Area of Science:

Microbiology
Environmental Science
Biotechnology

Background:

Sterility of cell products is vital, requiring controlled clean room environments.
Operator-generated particles are potential contaminants in aseptic manufacturing.
Environmental monitoring, including particle counts, is essential but operator impact needs clarification.

Purpose of the Study:

To assess the correlation between operator-generated particles and airborne bacteria.
To evaluate the effectiveness of different clothing types in reducing contamination.
To provide data for operator education and control methods in aseptic areas.

Main Methods:

Conducted accelerated tests in biosafety cabinets simulating human operations.
Monitored particle counts and airborne bacteria associated with operators wearing textile vs. dustless clothing.
Analyzed particle sizes and the percentage of bacteria within particles.

Main Results:

Textile clothing released significant particles and bacteria; dustless clothing did not.
A correlation was found between 5 μm particles and bacterial contamination with textile clothing.
Falling bacteria ratio was 0.8% for textile vs. 0.04% for dustless clothing.

Conclusions:

Clothing type directly influences particle and bacteria shedding.
Quantitative data can identify contamination risks and inform operator training.
Findings support evidence-based control strategies for aseptic manufacturing environments.