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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Updated: Jul 4, 2025

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing
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Fish cell line: depositories, web resources and future applications.

Murali S Kumar1, Vijay Kumar Singh1, Akhilesh Kumar Mishra1

  • 1ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh 226 002 India.

Cytotechnology
|February 2, 2024
PubMed
Summary
This summary is machine-generated.

Fish cell lines are vital bioresources for biological research and disease surveillance. Establishing cell line depositories ensures authenticated cell line availability for global researchers, aiding conservation efforts.

Keywords:
Biological modelCell linesDepositoryFishIn vitro

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Area of Science:

  • * Aquatic biology and cell line development.
  • * In vitro models for biological research and conservation.

Background:

  • * Cell lines are crucial bioresources for studying biological processes across various scientific disciplines.
  • * Fish cell lines derived from diverse organs serve as valuable in vitro models for disease surveillance, pharmacology, and metabolic studies.
  • * Global efforts have increased the development of new fish cell lines from various organs.

Purpose of the Study:

  • * To review the current status of fish cell lines in global depositories.
  • * To highlight the importance of cell line depositories for preserving and distributing authenticated cell lines.
  • * To emphasize the role of cell lines in conservation of aquatic life.

Main Methods:

  • * Literature review of existing fish cell line development and depository information.
  • * Analysis of the role of cell line depositories in facilitating cellular research.
  • * Discussion of cryopreservation and maintenance protocols for cell lines.

Main Results:

  • * A growing number of fish cell lines have been developed from various organs worldwide.
  • * The establishment of cell line depositories is essential for managing and providing access to these resources.
  • * Depositories ensure the availability of authenticated and characterized cell lines with established protocols.

Conclusions:

  • * Fish cell lines are indispensable tools for biological research and conservation.
  • * Cell line depositories play a critical role in supporting cellular research by ensuring access to reliable cell resources.
  • * Maintaining and distributing cell lines through depositories is vital for advancing scientific understanding and conservation efforts.