Related Experiment Video

Updated: Jul 4, 2025

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays
13:22

Kinase Inhibitor Screening In Self-assembled Human Protein Microarrays

Published on: October 23, 2019

7.9K

A kinase to cytokine explorer to identify molecular regulators and potential therapeutic opportunities

Marina Chan1, Yuqi Kang1, Shannon Osborne2

  • 1Human Biology Division, Fred Hutchinson Cancer Center, Seattle, United States.

Elife
|February 2, 2024
PubMed

Insights

This study introduces KinCytE, a web platform linking kinase inhibitors to cytokine and chemokine signaling. It helps identify drug targets for diseases like cancer by exploring kinase-cytokine networks.

Area of Science:

  • Biochemistry
  • Immunology
  • Pharmacology

Background:

  • Cytokines and chemokines are crucial secreted proteins regulating inflammation, immune responses, and cell differentiation.
  • Dysregulation of these signaling pathways is implicated in various diseases, notably cancer.
  • Targeting cytokines, chemokines, or their receptors is difficult due to pathway redundancy and complex signaling networks.

Purpose of the Study:

  • To develop a novel web-based platform, KinCytE, to facilitate the drugging of cytokine and chemokine targets.
  • To link the effects of kinase inhibitors with cytokine/chemokine release and signaling networks.
  • To provide a tool for investigating protein kinases regulating specific cytokines/chemokines and identifying potential therapeutic strategies.

Main Methods:

  • Development of the KinCytE web-based platform.
  • Integration of data linking protein kinases to cytokine and chemokine regulation.
  • Implementation of network visualization and inhibitor ranking functionalities.

Main Results:

  • KinCytE enables users to identify protein kinases that modulate specific cytokine or chemokine activity.
  • The platform generates a ranked list of FDA-approved kinase inhibitors impacting cytokine/chemokine activity.
  • Users can explore and visualize complex cytokine signaling networks.

Conclusions:

  • KinCytE transforms challenging cytokine and chemokine targets into a pharmacologically tractable class.
  • The platform aids in discovering and developing kinase inhibitors for cytokine-mediated diseases.
  • KinCytE offers a valuable resource for researchers investigating cytokine signaling and therapeutic interventions.
Keywords:
chemokinecomputational biologycytokinehumanimmunologyinflammationkinasemacrophagessignalingsystems biology

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