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Development and Validation of Anoiki-Related Lncrna Signature Prediction Model for KIRC Prognosis

  • 0College of Pharmacy, Chengdu University, Chengdu, China.
Combinatorial Chemistry & High Throughput Screening +

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February 2, 2024

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February 2, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study identifies four key anoikis-related long noncoding RNAs (lncRNAs) as powerful prognostic markers for kidney renal clear cell carcinoma (KIRC). These lncRNAs can predict patient outcomes and may offer insights into new KIRC treatment strategies.

Area Of Science

  • Oncology
  • Molecular Biology
  • Bioinformatics

Background

  • Long noncoding RNAs (lncRNAs) are implicated in various cancers.
  • Limited research exists on anoikis-related lncRNAs in kidney renal clear cell carcinoma (KIRC).
  • This study addresses the need for prognostic markers in KIRC by investigating anoikis-related lncRNAs.

Purpose Of The Study

  • To develop a prognostic model for KIRC patients using anoikis-related lncRNAs.
  • To identify potential therapeutic targets within these lncRNAs.
  • To explore the relationship between anoikis-related lncRNAs and the kidney immune microenvironment.

Main Methods

  • Identified prognostic anoikis-related lncRNAs using statistical analyses (Pearson correlation, Cox regression).
  • Constructed a predictive model with four lncRNAs via LASSO regression.
  • Assessed model performance using Kaplan-Meier and ROC analyses.
  • Evaluated immune cell infiltration and gene expression using ESTIMATE, CIBERSORT, TIMER, and QUANTISEQ.
  • Validated findings through drug sensitivity assays and in vitro experiments.

Main Results

  • Identified 63 anoikis-related lncRNAs, selecting four (Z99289.2, AC084876.1, LINC00460, AC090337.2) as hub markers.
  • Developed and validated a prognostic signature based on these four lncRNAs in TCGA KIRC cohort.
  • The signature accurately distinguished high-risk from low-risk KIRC patients and predicted outcomes.
  • In vitro experiments confirmed LINC00460 downregulation inhibits KIRC cell growth and promotes apoptosis.

Conclusions

  • Anoikis-related lncRNAs are promising prognostic markers for KIRC.
  • These lncRNAs may offer insights into KIRC treatment strategies.
  • The study highlights the potential of lncRNAs in understanding the KIRC immune microenvironment.

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