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Related Concept Videos

Protein Networks02:26

Protein Networks

4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
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High throughput methods to study protein-protein interactions during host-pathogen interactions.

Giridhar Chandrasekharan1, Meera Unnikrishnan1

  • 1Division of Biomedical Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom.

European Journal of Cell Biology
|February 2, 2024
PubMed
Summary

Understanding host-pathogen protein-protein interactions (PPIs) is crucial for infection outcomes. This review highlights high-throughput (HT) technologies for studying these complex host-bacterial PPIs.

Keywords:
High throughputHost-pathogen interactionsLaboratory-based techniquesMass spectrometryProtein-protein interaction

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Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Host-pathogen protein-protein interactions (PPIs) are critical determinants of infection.
  • These interactions can occur within or outside host cells, influencing infection severity.
  • Identifying these interactions is key to understanding pathogen survival and host response.

Purpose of the Study:

  • To review major high-throughput (HT) laboratory-based technologies for studying host-bacterial PPIs.
  • To address the complexity and diversity of host-pathogen interactions.
  • To provide an overview of unbiased methods for analyzing multiple interactions simultaneously.

Main Methods:

  • Review of existing literature on high-throughput (HT) technologies.
  • Focus on laboratory-based techniques for host-bacterial interaction studies.
  • Inclusion of biochemical, biophysical, and bioinformatic approaches.

Main Results:

  • Identification of several key high-throughput (HT) technologies applicable to host-bacterial PPI studies.
  • Discussion of the advantages of HT methods in handling the complexity of host-pathogen interactions.
  • Emphasis on the unbiased nature of these screening techniques.

Conclusions:

  • High-throughput (HT) technologies are essential for comprehensively studying host-bacterial protein-protein interactions (PPIs).
  • These advanced methods facilitate the analysis of numerous interactions, aiding in understanding infection dynamics.
  • The reviewed technologies offer powerful tools for future research in host-pathogen interactions.