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Updated: Jul 4, 2025

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Protocol to retrieve unknown flanking DNA sequences using semi-site-specific PCR-based genome walking.

Hong Chen1, Cheng Wei1, Zhiyu Lin2

  • 1State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, P.R. China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, P.R. China.

STAR Protocols
|February 3, 2024
PubMed
Summary

This study introduces a semi-site-specific primer PCR (3SP-PCR) method for identifying unknown DNA sequences. The protocol details primer design, experimental procedures, and data analysis for efficient DNA sequencing.

Keywords:
Biotechnology and bioengineeringGeneticsMolecular BiologySequencing

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