The interfacial interaction between typical microplastics and Pb²⁺ and their combined toxicity to Chlorella...

Microplastics (MPs), a new type of pollutant, have attracted much attention worldwide. MPs are often complexed with other pollutants such as heavy metals, resulting in combined toxicity to organisms in the environment. Studies on the combined toxicity of MPs and heavy metals have usually focused on the marine, while on the freshwater are lacking. In order to understand the combined toxic effects of MPs and heavy metals in the freshwater, five typical MPs (PVC, PE, PP, PS, PET) were selected to investigate the adsorption characteristics of MPs to Pb²⁺ before and after the MPs aging by ultraviolet (UV) irradiation through static adsorption tests. The results showed that UV aging enhanced adsorption of Pb²⁺ by MPs. It is noteworthy that MPs-PET had the highest adsorption capacity for Pb²⁺, and the interaction between MPs-PET and Pb²⁺ was the strongest. We specifically selected MPs-PET to study its combined toxicity with Pb²⁺ to Chlorella pyrenoidosa. In the combined toxicity test, MPs-PET and Pb²⁺ had significant toxic effects on Chlorella pyrenoidosa in the individual exposure, and the toxicity of individual Pb²⁺ exposure was greater than that of individual MPs-PET exposure. In the combined exposure, when MPs-PET and Pb²⁺ without adsorption (MPs-PET/Pb²⁺), MPs-PET and Pb²⁺ had a synergistic effect, which would produce strong physical and chemical stress on Chlorella pyrenoidosa simultaneously, and the toxic effect was the most significant. After the adsorption of MPs-PET and Pb²⁺ (MPs-PET@Pb²⁺), the concentration and activity of Pb²⁺ decreased due to the adsorption and fixation of MPs-PET, and the chemical stress on Chlorella pyrenoidosa was reduced, but the physical stress of MPs-PET still existed and posed a serious threat to the survival of Chlorella pyrenoidosa. This study has provided a theoretical basis for further assessment of the potential environmental risks of MPs in combination with other pollutants such as heavy metals.