Related Experiment Video
Updated: Jul 4, 2025

A Simple and Rapid Protocol for Measuring Neutral Lipids in Algal Cells Using Fluorescence
Published on: May 30, 2014
Rapid quantitative evaluation of total polar materials (TPM) in frying oil based on an "off-on" fluorescence
Jiayi Shi1, Jian Tao2, Yanfeng Fu3
1College of Chemistry, Henan Joint International Research Laboratory of Green Construction of Functional Molecules and Their Bioanalytical Applications, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Laboratory of Zhongyuan Food, Zhengzhou University, Zhengzhou, 450001, China.
Abstract:
The content of total polar material (TPM) is considered as a comprehensive indicator to evaluate the quality of edible oils which should be discarded and no longer be used when TPM content exceeding 27 %. Nevertheless, there is currently a lack of a convenient and efficient TPM detection method, which is a meaningful challenge. With the increase of TPM content, the viscosity of frying oil grows, and the two maintain a satisfactory positive correlation. Consequently, an "off-on" fluorescence probe TCF-PR method based on viscosity-response has been developed. There exists a good linear relationship between the fluorescence intensity of the probe and the TPM content of soybean oil ((R2 = 0.9936) and salad oil (R2 = 0.9878), accompanying with the advantage of fast response (3 s), which means the rapid detection of TPM can be realized to determine the quality of frying oil in the field of food safety.

