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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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Regulation of Angiogenesis and Blood Supply01:24

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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The Nucleolus02:55

The Nucleolus

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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
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Expression and clinical significance of hypoxia-induced long non-coding RNA TCONS_I2_00001955 in breast cancer.

Jie-Yin Zhuang1,2, Ze-Nan Huang3, Zi-Jin Weng4

  • 1Department of Medical Oncology, The Third Affiliated Hospital of Sun Yat-Sen University, 600 Tianhe Road, Guangzhou, 510630, China.

Breast Cancer (Tokyo, Japan)
|February 4, 2024
PubMed
Summary
This summary is machine-generated.

Hypoxia promotes breast cancer metastasis via long non-coding RNA TCONS_I2_00001955. This lncRNA is linked to aggressive features and poor patient survival in invasive breast cancer.

Keywords:
Breast cancerHypoxiaMetastasislncRNA TCONS_I2_00001955

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in tumor development and metastasis.
  • Investigating lncRNAs under hypoxia is crucial for understanding breast cancer progression.

Purpose of the Study:

  • Screen for lncRNAs promoting breast cancer cell invasion and metastasis under hypoxia.
  • Examine the relationship between lncRNA expression, clinicopathological features, and prognosis in invasive breast cancer.

Main Methods:

  • Utilized lncRNA microarray and qRT-PCR to screen and verify differentially expressed lncRNAs in breast cancer cell lines under hypoxia.
  • Employed CCK8 and Transwell assays to assess the functional impact of lncRNAs on cell proliferation, migration, and invasion.
  • Analyzed lncRNA and HIF-1α expression in patient samples using RNAscope and immunohistochemistry, correlating with clinical data and survival outcomes via Cox regression.

Main Results:

  • lncRNA TCONS_I2_00001955 expression was upregulated in breast cancer cells under hypoxia.
  • Overexpression of TCONS_I2_00001955 significantly increased proliferation, migration, and invasion.
  • Positive TCONS_I2_00001955 expression correlated with recurrence, metastasis, and high HIF-1α levels, identifying it as an independent risk factor for poor disease-free survival.

Conclusions:

  • Hypoxia-induced lncRNA TCONS_I2_00001955 is associated with aggressive breast cancer phenotypes.
  • TCONS_I2_00001955 may serve as a potential biomarker for predicting poor prognosis in invasive breast cancer.