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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
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  9. Predictive And Prognostic Markers
  11. Expression And Clinical Significance Of Hypoxia-induced Long Non-coding Rna Tcons_i2_00001955 In Breast Cancer

Expression and clinical significance of hypoxia-induced long non-coding RNA TCONS_I2_00001955 in breast cancer

Jie-Yin Zhuang1,2, Ze-Nan Huang3, Zi-Jin Weng4

  • 1Department of Medical Oncology, The Third Affiliated Hospital of Sun Yat-Sen University, 600 Tianhe Road, Guangzhou, 510630, China.

Breast Cancer (Tokyo, Japan)
|February 4, 2024

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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
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Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

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Induction and Testing of Hypoxia in Cell Culture
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Induction and Testing of Hypoxia in Cell Culture

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View abstract on PubMed

Summary
This summary is machine-generated.

Hypoxia promotes breast cancer metastasis via long non-coding RNA TCONS_I2_00001955. This lncRNA is linked to aggressive features and poor patient survival in invasive breast cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in tumor development and metastasis.
  • Investigating lncRNAs under hypoxia is crucial for understanding breast cancer progression.

Purpose of the Study:

  • Screen for lncRNAs promoting breast cancer cell invasion and metastasis under hypoxia.
  • Examine the relationship between lncRNA expression, clinicopathological features, and prognosis in invasive breast cancer.

Main Methods:

  • Utilized lncRNA microarray and qRT-PCR to screen and verify differentially expressed lncRNAs in breast cancer cell lines under hypoxia.
  • Employed CCK8 and Transwell assays to assess the functional impact of lncRNAs on cell proliferation, migration, and invasion.
  • Analyzed lncRNA and HIF-1α expression in patient samples using RNAscope and immunohistochemistry, correlating with clinical data and survival outcomes via Cox regression.
Keywords:
Breast cancerHypoxiaMetastasislncRNA TCONS_I2_00001955

Related Experiment Videos

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
09:17

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Published on: August 2, 2018

18.2K
Induction and Testing of Hypoxia in Cell Culture
07:01

Induction and Testing of Hypoxia in Cell Culture

Published on: August 12, 2011

85.0K
Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

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Main Results:

  • lncRNA TCONS_I2_00001955 expression was upregulated in breast cancer cells under hypoxia.
  • Overexpression of TCONS_I2_00001955 significantly increased proliferation, migration, and invasion.
  • Positive TCONS_I2_00001955 expression correlated with recurrence, metastasis, and high HIF-1α levels, identifying it as an independent risk factor for poor disease-free survival.

Conclusions:

  • Hypoxia-induced lncRNA TCONS_I2_00001955 is associated with aggressive breast cancer phenotypes.
  • TCONS_I2_00001955 may serve as a potential biomarker for predicting poor prognosis in invasive breast cancer.