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Updated: Jul 4, 2025
12:00
Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
Published on: April 8, 2009
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Genomic and transcriptomic analysis of the recent Mpox outbreak
Federico M Giorgi1, Daniele Pozzobon1, Antonio Di Meglio1
1Department of Pharmacy and Biotechnology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy.
Vaccine
|February 4, 2024
Summary
The recent Mpox outbreak likely originated from the West African clade, showing high genomic similarity to historical Mpox, Cowpox, and Variola viruses. This suggests conserved immune responses and potential vaccine cross-protection.
Area of Science:
- Virology
- Genomics
- Immunology
Background:
- The Mpox (formerly Monkeypox) virus caused a multi-country outbreak starting in 2022.
- Understanding the genomic origins and relatedness of Mpox is crucial for public health.
Purpose of the Study:
- To analyze Mpox virus genomes from the recent outbreak and compare them with historical Mpox, Cowpox, and Variola virus sequences.
- To investigate genomic conservation and transcriptional responses across different Poxviridae family members.
Main Methods:
- Full genome sequencing of Mpox virus from Europe.
- Comparative genomic analysis with historical Mpox, Cowpox, and Variola virus sequences.
- Analysis of human transcriptional responses in cell cultures exposed to Poxviruses.
Main Results:
- The recent Mpox outbreak is linked to the West African clade with >99% sequence identity to historical strains.
- High genomic conservation (97.8-97.9%) was observed between Mpox, Cowpox, and Variola viruses.
- A conserved immune pathway response was identified across these Poxvirus families in human cell cultures.
Conclusions:
- Genomic and transcriptomic similarities provide a molecular basis for the efficacy of Variola vaccines against other Poxviruses.
- The findings support the close evolutionary relationship between Mpox, Cowpox, and Variola viruses.
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