Naringenin protects myocardial ischemia/reperfusion injury by regulating miR-24-3p to inhibit cell death-inducing p53 target 1 expression
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Xiao Jin1, Ling Jin1, Bingxin Wu2
1Department of Chinese Medicine, The First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, P. R. China.
Abstract:
Myocardial ischemia/reperfusion (I/R) causes serious threats to human life. Naringenin, a polyphenolic compound naturally occurring in citrus fruit, has cardioprotective effects against myocardial I/R injury. Besides, miR-24-3p is also reported to have cardioprotective effects. We intended to explore whether the cardioprotective effects of naringenin relate to miR-24-3p and its underlying mechanism. In this study, we used an in vivo rat myocardial I/R model and an in vitro cardiomyocyte H9c2 hypoxia/reoxygenation (H/R) model. Myocardial injury was detected by hematoxylin-eosin staining and ELISA for creatine kinase (CK), malondialdehyde (MDA), and lactate dehydrogenase (LDH). miR-24-3p and cell death inducing p53 target 1 (Cdip1) mRNA expressions were examined by RT-PCR. We find that naringenin pretreatment significantly relieves myocardial I/R injury, reduces LDH, CD, and MDA levels, and increases miR-24-3p expression. Furthermore, miR-24-3p alleviates myocardial I/R injury partially through regulating Cdip1. Moreover, naringenin protects myocardial I/R injury partially by regulating miR-24-3p to inhibit Cdip1 expression. In conclusion, our data suggest naringenin protects myocardial I/R injury partially through miR-24-3p/Cdip1 axis.
Insights
Naringenin, a citrus compound, protects against heart injury from ischemia/reperfusion (I/R) by increasing miR-24-3p. This microRNA helps reduce cell death by regulating Cdip1 expression, offering a potential therapeutic pathway.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Pharmacology
Background:
- Myocardial ischemia/reperfusion (I/R) injury poses significant health risks.
- Naringenin, a citrus polyphenol, demonstrates cardioprotective properties.
- MicroRNA-24-3p (miR-24-3p) is also recognized for its role in protecting the heart.
Purpose of the Study:
- To investigate the potential link between naringenin's cardioprotective effects and miR-24-3p.
- To elucidate the underlying molecular mechanisms involved in naringenin's action.
Main Methods:
- Utilized in vivo rat myocardial I/R and in vitro H9c2 cell hypoxia/reoxygenation (H/R) models.
- Assessed myocardial injury using histology and biochemical markers (CK, MDA, LDH).
- Quantified miR-24-3p and Cdip1 mRNA expression via RT-PCR.
Main Results:
- Naringenin pretreatment significantly reduced myocardial I/R injury, decreasing LDH, CK, and MDA levels.
- Naringenin administration led to increased expression of miR-24-3p.
- miR-24-3p was found to partially alleviate I/R injury by regulating Cdip1 expression.
Conclusions:
- Naringenin exerts cardioprotective effects against I/R injury, partly mediated by the miR-24-3p/Cdip1 axis.
- The findings suggest naringenin inhibits Cdip1 expression through miR-24-3p, offering a novel therapeutic strategy for myocardial I/R injury.
