Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single cell analysis of commitment in early embryogenesis.

J Heasman, A Snape, J Smith

    Journal of Embryology and Experimental Morphology
    |November 1, 1985
    PubMed
    Summary

    Cell determination in amphibian embryos is a gradual process. New single-cell labeling techniques reveal endoderm commitment begins mid-blastula and completes by gastrulation, offering insights into developmental mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Ethnic differences in cross-sectional associations between impaired glucose regulation, identified by oral glucose tolerance test or HbA1c values, and cardiovascular disease in a cohort of European and South Asian origin.

    Diabetic medicine : a journal of the British Diabetic Association·2015
    Same author

    A plant selectable marker gene based on the detoxification of the herbicide dalapon.

    Plant cell reports·2013
    Same author

    What my mother told me: Examining the roles of maternal gene products in a vertebrate.

    Trends in cell biology·2007
    Same author

    The role of FoxC1 in early Xenopus development.

    Developmental dynamics : an official publication of the American Association of Anatomists·2007
    Same author

    Vegetal localization of maternal mRNAs is disrupted by VegT depletion.

    Developmental biology·2002
    Same author

    Timing of endogenous activin-like signals and regional specification of the Xenopus embryo.

    Development (Cambridge, England)·2001

    Area of Science:

    • Developmental Biology
    • Cellular Biology
    • Embryology

    Background:

    • Amphibian fate maps historically indicated distinct germ layer regions by the late blastula stage.
    • Previous explant experiments suggested ectoderm, mesoderm, and endoderm were determined by the late blastula stage.
    • Spemann questioned whether early embryonic regions were pre-destined or indifferent until later determination.

    Purpose of the Study:

    • To precisely determine the timing of cell commitment during early amphibian embryogenesis.
    • To investigate the gradual process of endoderm determination in the vegetal pole.
    • To enable molecular-level studies of cell determination mechanisms by comparing committed and uncommitted states.

    Main Methods:

    • Utilized a novel method combining single-cell labeling and transplantation techniques.
    • Enabled precise tracking of individual blastomere fates during early development.
    • Allowed for detailed temporal analysis of cell commitment events.

    Main Results:

    • Endoderm determination in the vegetal pole is a gradual process, not an abrupt event.
    • Commitment towards endoderm begins during the middle blastula stage (stage 8).
    • Endoderm determination is completed by the onset of gastrulation (stage 10).

    Conclusions:

    • Cellular determination in amphibian embryos is a progressive, stage-dependent process.
    • The vegetal pole cells gradually commit to an endodermal fate.
    • This research provides a foundation for molecular investigations into the mechanisms of cell determination.

    Related Experiment Videos