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Updated: Jul 4, 2025
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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
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Modularly engineering Rhodotorula toruloides for α-terpineol production.
Liting Lyu1,2, Qiongqiong Chen1,3, Haizhao Xue1,3
1Laboratory of Biotechnology, Dalian Institute of Chemical Physics, Dalian, China.
Frontiers in Bioengineering and Biotechnology
|February 5, 2024
Summary
Researchers engineered the yeast Rhodotorula toruloides for direct biosynthesis of α-terpineol. This microbial production achieved 1.5 mg/L, demonstrating a new route for producing this valuable monoterpenoid alcohol.
Area of Science:
- Biotechnology
- Synthetic Biology
- Microbial Engineering
Background:
- α-Terpineol is a monoterpenoid alcohol with applications in flavor, fragrance, and pharmaceuticals.
- Microbial production of α-terpineol remains underexplored.
- Rhodotorula toruloides possesses a natural mevalonate pathway, making it a suitable host for biosynthesis.
Purpose of the Study:
- To engineer Rhodotorula toruloides for direct biosynthesis of α-terpineol.
- To enhance monoterpenoid production through modular engineering strategies.
- To establish R. toruloides as a viable host for terpenoid synthesis.
Main Methods:
- Enhancement of precursor supply for α-terpineol biosynthesis.
- Blocking of downstream metabolic pathways to prevent product degradation.
- Disruption of competing metabolic pathways to redirect resources.
- Engineering of R. toruloides using modular strategies.
Main Results:
- Modular engineering strategies yielded varying improvements in α-terpineol production.
- The engineered strain 5L6HE5 achieved a peak α-terpineol titer of 1.5 mg/L.
- Reducing lipid accumulation in R. toruloides enhanced terpenoid synthesis.
Conclusions:
- Rhodotorula toruloides can be engineered for efficient microbial production of α-terpineol.
- Disrupting competing pathways and increasing substrate supply are effective strategies.
- This study highlights the potential of R. toruloides for synthesizing various monoterpenoids.
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