Methods of Protection of Electrochemical Sensors against Biofouling in Cell Culture Applications

In this work, we evaluated more than 10 antifouling layers presenting different modes of action for application in electrochemical sensors. These layers included porous materials, permselective membranes, hydrogels, silicate sol-gels, proteins, and sp³ hybridized carbon. To evaluate the protective effects of the antifouling modification as well as its impact on the catalyst, we adsorbed a redox mediator on the electrode surface. Five of the tested coatings allowed us to preserve the electrochemical properties of the tested mediator. Later studies showed that sol-gel silicate layer, poly-l-lactic acid, and poly(l-lysine)-g-poly(ethylene glycol) were the only ones capable of sustaining the catalyst's performance during prolonged incubation in a cell culture medium. The highest signal deterioration was observed, as expected during the first few hours of incubation in a cell culture environment. Tested layers exhibited different dynamics of the protective effect. The poly-l-lactic acid layer presented lower changes in the first hours of the study but suffered complete signal deterioration after 72 h. Whereas the signal intensity of the silicate layer was lowered by half after just 3 h but was still visible after 6 weeks of constant incubation of the electrode in the cell culture.