Related Experiment Video
Updated: Jul 4, 2025
Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
A fluorescent aptasensor for highly sensitive and selective detection of carcinoembryonic antigen based on
Yunxia Yuan1, Yi Di2, Yuan Chen1
1College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. yinyong@haust.edu.cn.
Abstract:
A highly sensitive fluorescent aptasensor for carcinoembryonic antigen (CEA) was developed by employing upconversion nanoparticles (UCNPs) as an energy donor and WS2 nanosheets as an energy acceptor, respectively. Polyacrylic acid (PAA) modified NaYF4:Yb/Er UCNPs and an amine modified CEA aptamer were linked together by a covalent bond. Owing to the physical adsorption between WS2 nanosheets and the CEA aptamer, the UCNPs-aptamer was close to WS2 nanosheets, resulting in upconversion fluorescence energy transfer from UCNPs to WS2 nanosheets, and the UCNP fluorescence was quenched. With the introduction of CEA into the UCNPs-aptamer complex system, the aptamer preferentially bound to CEA resulting in a change in spatial conformation which caused UCNPs to depart from WS2 nanosheets. As a result, the energy transfer was inhibited and the fluorescence of UCNPs was observed again, and the degree of fluorescence recovery was linearly related to the concentration of CEA in a range of 0.05-10 ng mL-1 with a limit of detection of 0.008 ng mL-1. Furthermore, the aptasensor based on UCNPs and WS2 nanosheets could be competent for detecting CEA in human serum, which suggests the great application potential of the proposed aptasensor in clinical diagnosis.
