Diethylnitrosamine-Induced Liver Tumorigenesis in Mice Under High-Hat High-Sucrose Diet: Stepwise High-Resolution Ultrasound Imaging and Histopathological Correlations
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View abstract on PubMed
Summary
This summary is machine-generated.Diethylnitrosamine (DEN) induces liver neoplasms in mice, mimicking human liver cancer development. This model uses imaging to track tumor growth and study cancer modifiers.
Area Of Science
- Hepatocarcinogenesis research
- Preclinical cancer models
- Liver tumor development
Background
- Hepatocellular carcinoma (HCC) is a multi-step process driven by chronic liver injury and inflammation.
- The hepatotoxic compound diethylnitrosamine (DEN) reliably induces liver neoplasms in rodents.
- The DEN model recapitulates key stages of human hepatocarcinogenesis, including initiation, promotion, and progression.
Purpose Of The Study
- To provide a detailed protocol for inducing hepatocellular neoplasms in mice using DEN and a high-fat, high-sucrose (HFHS) diet.
- To highlight the utility of ultrasound liver imaging for monitoring tumor development in vivo.
- To discuss factors influencing liver tumorigenesis within this preclinical model.
Main Methods
- Administration of diethylnitrosamine (DEN) intraperitoneally to 14-day-old C57BL/6J male mice.
- Feeding mice a high-fat, high-sucrose (HFHS) diet for 36 weeks to promote tumorigenesis.
- Utilizing high-resolution ultrasound liver imaging to track tumor progression and correlating findings with histopathology.
Main Results
- Successful induction of hepatocellular neoplasms in mice following the described protocol.
- Demonstration of ultrasound imaging's effectiveness in monitoring tumor development over time.
- Identification of histopathological changes consistent with multistage hepatocarcinogenesis.
Conclusions
- The DEN-induced liver tumorigenesis model is a valuable preclinical tool for studying HCC development.
- Ultrasound imaging provides a non-invasive method for real-time monitoring of liver tumor progression.
- This protocol facilitates research into genetic, dietary, and environmental modifiers of liver cancer.
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