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Corneal Donor Tissue Preparation for Endothelial Keratoplasty
Published on: June 12, 2012
Bowman Layer Marking for Correct Placement of Corneal Allogeneic Intrastromal Ring Segments
Soosan Jacob1,2, Amar Agarwal1, Shady Awwad3
1Dr. Agarwal's Eye Hospital and Eye Research Centre, Chennai, India.
Abstract:
Corneal allogeneic intrastromal ring segments (CAIRS) refer to the placement of allogeneic rings and segments in intrastromal channels within the cornea. Currently, a deepithelialized donor cornea is used as the allogeneic source for CAIRS and the cut ring is turned sideways and implanted so that the thickness can be varied by varying the distance between the concentric trephine blades. In addition, to obtain a greater effect, CAIRSs are preferred to be implanted with the Bowman layer (BL) facing the corneal apex and posterior stroma facing limbally. Being flexible tissue, it is, however, important to prevent twisting and to maintain correct orientation. We describe a simple technique of marking the BL with a gentian violet surgical marker to simplify CAIRS insertion. BL marking allows easy visibility of twists and helps identify improper orientation, thus allowing correct insertion of CAIRS.
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