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Evaluation of pathogen from the FilmArray meningitis/encephalitis panel and recommendations on atypical findings

Luiz Gustavo Ferreira Côrtes1, Mariana Menezes Maldonado1, Paula Celia Mariko Koga1

  • 1Hospital Israelita Albert Einstein, Laboratório Clínico, São Paulo SP, Brazil.

Arquivos De Neuro-Psiquiatria
|February 5, 2024
PubMed
Abstract

Insights

Reproducing atypical results from the FilmArray meningitis/encephalitis panel is effective. This approach helps interpret discordant findings, particularly bacterial detections, improving diagnostic accuracy for infectious meningoencephalitis.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Molecular Diagnostics

Background:

  • Infectious meningoencephalitis poses a significant threat, causing central nervous system inflammation due to various microorganisms.
  • The FilmArray meningitis/encephalitis panel offers rapid, simultaneous detection of 14 key pathogens.

Purpose of the Study:

  • To retrospectively evaluate the implementation and performance of the FilmArray meningitis/encephalitis panel in a hospital setting.
  • To analyze the consistency of panel results against clinical and laboratory data.

Main Methods:

  • Retrospective analysis of results from the BioFire FilmArray system's meningitis/encephalitis panel.
  • Correlated laboratory tests included biochemical, cytological, and microbiological analyses.
  • Investigation of discordant results through retesting and clinical-epidemiological review.

Main Results:

  • Out of 496 samples, 88 (17.75%) were positive, detecting 90 pathogens, with viruses being the most common.
  • 20 samples (4.03%) required retesting due to invalid results, multiple pathogen detection, or discordant findings.
  • 80% of retested samples with initial atypical results were non-reproducible, often linked to bacterial detections inconsistent with clinical data.

Conclusions:

  • Retesting atypical results from the FilmArray meningitis/encephalitis panel is an effective method for result interpretation.
  • This approach is crucial for resolving discrepancies and ensuring accurate diagnosis of infectious meningoencephalitis.

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