Vanillin-Mediated Green-Synthesised Silver Nanoparticles' Characterisation and Antimicrobial Activity: An In-Vitro
Daphane Anishya1, Ravindra Kumar Jain1
1Orthodontics and Dentofacial Orthopedics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IND.
Abstract:
Background and aim Nanoparticles in general due to their enhanced antimicrobial effects and other beneficial effects are used in dentistry. Silver nanoparticles (AgNPs) have emerged as the metal nanoparticle with the most advantages among the many types. The objective of the study was to synthesise vanillin-mediated AgNPs, then characterise those nanoparticles and assess their antimicrobial effectiveness. Materials and methods One-step synthesis of stable and crystalline AgNPs was done with vanillin as the reducing and capping agent. After being crushed into powder form, the produced AgNPs were subjected to characterisation. A scanning electron Microscope SEM) analysis was done for morphological details of the AgNPs. SEM with energy dispersive X-ray spectroscopy analysis (EDAX) and Fourier transform infrared (FTIR) testing were done for elemental analysis. AgNPs' antimicrobial properties were tested using the agar well diffusion technique. Results The SEM analysis revealed that the synthesized AgNps were porous and agglomerative clusters and varied in sizes between 30-35 nm. SEM-EDAX revealed the presence of 76.2 weight (wt)% Ag, 4.9 wt% carbon, and 18.9 wt% of oxygen. FTIR prominent peaks were observed at 1431.97 cm and 1361.20 cm indicating the presence of AgNPs. Both low and high concentrations of AgNps showed good antimicrobial effects against Streptococcus mutans (S. mutans). Conclusion Vanillin can be successfully used as a reducing agent for creating AgNPs. Due to their effective antimicrobial activity against S.mutans at various concentrations, vanillin-mediated AgNPs can be used with dental materials to reduce the risk of dental caries and enamel demineralization.
