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High Mobility Group Box 1 Gene Polymorphism and Serum High Mobility Group Box 1, Interleukin 1 Beta, and Alpha-Klotho
Maha Houssen1, Rasha El-Mahdy2, Nouran E Samra2
1Biochemistry Department, Faculty of Pharmacy, Damanhour University, Damnhour, Egypt.
Immunological Investigations
|February 6, 2024
Summary
Serum levels of HMGB1, IL1β, and α-klotho are elevated in critically ill COVID-19 patients. These biomarkers, along with HMGB1 rs1045411 polymorphism, may indicate disease severity and offer therapeutic targets.
Area of Science:
- Biochemistry
- Immunology
- Genetics
Background:
- COVID-19 severity varies, necessitating biomarkers for prognosis.
- HMGB1, IL1β, and α-klotho are implicated in inflammatory and cellular processes.
Purpose of the Study:
- Evaluate serum HMGB1, IL1β, and α-klotho in COVID-19 patients across severity spectrums.
- Correlate these markers with clinicopathological parameters.
- Assess HMGB1 rs1045411 polymorphism and its association with COVID-19 severity.
Main Methods:
- Serum samples from 120 COVID-19 patients and 80 controls were analyzed.
- HMGB1, IL1β, and α-klotho levels measured using ELISA.
- HMGB1 rs1045411 polymorphism genotyped via RT-PCR.
Main Results:
- Significantly higher serum HMGB1, IL1β, and α-klotho in critically ill COVID-19 patients.
- HMGB1 rs1045411 polymorphism showed decreased T/T genotype frequency in patients versus controls.
- ROC analysis indicated moderate diagnostic utility for these serum markers.
Conclusions:
- Serum HMGB1, IL1β, and α-klotho may serve as indicators of COVID-19 severity.
- These biomarkers represent potential therapeutic targets for COVID-19 management.
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