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Updated: Apr 29, 2026

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Published on: April 14, 2016

A novel method for sampling subgingival microbiome: a comparative metatranscriptomic study.

Diana Demusaj¹, Ryan Toma¹, Tanveer Khan¹

¹Viome Life Sciences, Inc., Bothell, WA 98011 and Bellevue, WA 98004, New York, NY 10018, USA.

|February 6, 2024

Summary

This summary is machine-generated.

A new proxy sampling method for the subgingival microbiome (subgingival-P samples) accurately reflects microbial composition and gene expression. This technique simplifies subgingival microbiome analysis for large-scale health and disease studies.

Keywords:

RNA metatranscriptomics oral microbiome sample collection subgingival supragingival

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Area of Science:

Microbiology
Oral Health
Genomics

Background:

The subgingival microbiome plays a role in oral and systemic diseases.
Current subgingival microbiome sampling methods are challenging.

Purpose of the Study:

To develop and validate a novel, less invasive method for subgingival microbiome sampling.
To assess the utility of subgingival-P samples as a proxy for direct subgingival samples.

Main Methods:

Developed a novel rotational swabbing technique on the supragingival area to collect subgingival-P samples.
Performed metatranscriptomic analysis on both subgingival and subgingival-P samples from 20 individuals.
Compared microbial relative abundances and gene expression profiles between the two sample types.

Main Results:

Subgingival-P samples showed comparable microbial relative abundances to direct subgingival samples.
Microbial gene expression levels were also similar between subgingival and subgingival-P samples.
The novel method demonstrated high concordance with traditional subgingival sampling.

Conclusions:

The subgingival-P sampling method is a viable proxy for subgingival microbiome analysis.
This technique facilitates larger and more diverse studies on the subgingival microbiome's role in health and disease.
Enables easier investigation into conditions like periodontitis and Alzheimer's disease.