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Updated: May 2, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
Published on: May 3, 2011
Photoacoustic Lifetime Imaging of Hypoxia
Qi Shao1, Joshua G Punnoose2,3
1Department of Radiology, University of Minnesota, Minneapolis, MN, USA. shaox070@umn.edu.
Abstract:
We present an approach based on photoacoustic lifetime imaging (PALI) to map the distribution of oxygen partial pressure (pO2) in tissue. This method utilizes methylene blue, a dye widely used in clinical applications, as an oxygen-sensitive imaging agent. PALI measurement of oxygen relies upon pO2-dependent excitation lifetime of the dye. The technique maps the excited triplet state of oxygen-sensitive dye, thus reflecting the spatial and temporal distributions of tissue oxygen.
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