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Staining Hypoxic Areas of Frozen and FFPE Tissue Sections with Hypoxyprobe™

Harsh H Oza1, Esther Ng2, Daniele M Gilkes3

  • 1Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|February 6, 2024
PubMed

Keywords:
FFPE tissueFrozen tissueHypoxiaHypoxyprobe™IFIHCImmunofluorescence stainingImmunohistochemistryMouse samplesPimonidazole hydrochloride

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Fixation and Sectioning01:03

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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...