Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.6K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.6K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.7K
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

4.6K
The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the...
4.6K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Self-sustained heterogeneity drives PDAC.

Science signaling·2026
Same author

An inflammation memento.

Science signaling·2026
Same author

Fungi facilitate gut healing.

Science signaling·2026
Same author

<i>Shigella</i> mixes and matches host proteins.

Science signaling·2025
Same author

Sensory support for stem cells.

Science signaling·2025
Same author

Estrogen-powered kidney protection.

Science signaling·2025

Related Experiment Video

Updated: Jul 4, 2025

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.6K

Extracellular WNT conveyors.

Annalisa M VanHook1

  • 1Science Signaling, AAAS, Washington, DC 20005, USA.

Science Signaling
|February 6, 2024
PubMed
Summary

Secreted frizzled-related proteins (SFRP) and WNT inhibitory factor 1 (WIF1) are extracellular proteins that bind to WNT ligands. These proteins play crucial roles in modulating WNT signaling pathways, affecting cellular processes.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Developmental Biology

Background:

  • WNT signaling is a critical pathway in embryonic development and adult tissue homeostasis.
  • Dysregulation of WNT signaling is implicated in various diseases, including cancer.
  • Extracellular modulators of WNT signaling are key targets for therapeutic intervention.

Purpose of the Study:

  • To investigate the distinct roles of SFRP and WIF1 in WNT signaling.
  • To elucidate the mechanisms by which SFRP and WIF1 exert their regulatory functions.
  • To understand the implications of SFRP and WIF1 activity in biological contexts.

Main Methods:

  • Biochemical assays to assess WNT-ligand binding.
  • Cell-based reporter assays to measure WNT pathway activity.

More Related Videos

Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.4K
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.4K

Related Experiment Videos

Last Updated: Jul 4, 2025

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

1.6K
Studying Wnt Signaling During Patterning of Conducting Airways
13:00

Studying Wnt Signaling During Patterning of Conducting Airways

Published on: October 16, 2016

7.4K
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.4K
  • In vitro and in vivo models to study protein function.

    • Main Results:

    • SFRP proteins were found to act as WNT antagonists by sequestering WNT ligands.
    • WIF1 also functions as a WNT inhibitor, preventing ligand interaction with receptors.
    • Differential expression and activity of SFRP and WIF1 contribute to the fine-tuning of WNT signaling outcomes.

    Conclusions:

    • SFRP and WIF1 are essential extracellular regulators of WNT signaling.
    • Targeting SFRP and WIF1 offers potential therapeutic strategies for WNT-dependent diseases.
    • Further research into these WNT-binding proteins will advance our understanding of developmental and disease processes.