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Basic Transcription Factor 3 Like 4 Enhances Malignant Phenotypes through Modulating Tumor Cell Function and Immune
Bing Lu1, Tianyi Lu2, Jiawen Shi1
1Department of Clinical Biobank and Institute of Oncology, Affiliated Hospital of Nantong University, Jiangsu, China.
The American Journal of Pathology
|February 6, 2024
Summary
Basic transcription factor 3 like 4 (BTF3L4) is elevated in glioma, correlating with poor prognosis and influencing tumor cell behavior. Its association with immune cells suggests potential as a target for glioma immunotherapy.
Area of Science:
- Neuro-oncology
- Cancer immunology
- Molecular biology
Background:
- Glioma immunotherapy response is limited by the tumor microenvironment.
- The role of basic transcription factor 3 like 4 (BTF3L4) in glioma and its immune cell infiltration is unclear.
Purpose of the Study:
- To investigate BTF3L4 expression in glioma.
- To assess its prognostic value and correlation with immune cell infiltration.
- To explore its functional role in glioma cell behavior.
Main Methods:
- Gene expression profiling and immunohistochemistry for BTF3L4.
- Prognostic analysis using Cox regression and Kaplan-Meier methods.
- In vitro experiments on glioma cell lines.
- Immune cell quantification (CIBERSORT, ESTIMATE) and multiplex immunohistochemistry.
Main Results:
- BTF3L4 expression is higher in glioma than non-tumor tissues, linked to adverse clinical characteristics and prognosis.
- Downregulation of BTF3L4 impairs glioma cell proliferation, migration, and invasion.
- BTF3L4 correlates with CD66B+ neutrophil infiltration and PD-L1 expression in glioma.
Conclusions:
- BTF3L4 has significant prognostic value in glioma.
- BTF3L4 influences glioma cell aggressiveness.
- BTF3L4 is associated with specific immune infiltrates and may be a therapeutic target for glioma immunotherapy.
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