Imprinted covalent organic frameworks solid-phase microextraction fiber for in vivo monitoring of acidic per- and polyfluoroalkyl substances in live aloe

Lidan Zhang ¹, Haoyue Han ¹, Jing Zhou ²

⁰Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China.

The Science of the Total Environment +

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February 6, 2024

View abstract on PubMed

Summary

A new method allows for in vivo monitoring of per- and polyfluoroalkyl substances (PFASs) in live plants. This research provides crucial data on PFAS uptake and translocation in plants, aiding agricultural safety assessments.

Area Of Science

Environmental Chemistry
Analytical Chemistry
Plant Science

Background

Per- and polyfluoroalkyl substances (PFASs) pose health risks via food chain transfer.
PFASs are absorbed by plant roots and translocated throughout plant tissues.
Accurate in vivo monitoring in plants is needed to understand PFAS exposure mechanisms.

Purpose Of The Study

Develop an analytical method for in vivo monitoring of PFASs in live plants.
Quantify PFAS uptake, translocation, and elimination dynamics in plants.
Assess the agricultural production safety risks associated with PFAS contamination.

Main Methods

Developed a novel imprinted covalent organic frameworks (CMIP) solid-phase microextraction technique.
Coupled CMIP with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for sensitive detection.
Utilized the method for in vivo monitoring of six acidic PFASs in live aloe plants grown in contaminated soil.

Main Results

Achieved low limits of detection for six acidic PFASs (0.1-0.3 ng g⁻¹).
Demonstrated high extraction efficiency and good precision (intra- and inter-RSD ≤10.2%) with the CMIP coating.
Obtained vital in vivo data including steady-state concentrations, translocation factors, elimination rate constants, and half-life of PFASs.

Conclusions

The developed CMIP-based in vivo method enables precise monitoring of PFASs in live plants.
This technique provides essential data for evaluating PFAS risks in the food chain and agricultural safety.
The findings are critical for ensuring the safety of agricultural production and mitigating environmental contamination.

Keywords:

Covalent organic frameworks In vivo tracing Molecular imprinting Per- and polyfluoroalkyl substances Solid-phase microextraction