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Bioinformatics identification of potential biomarkers and therapeutic targets for ischemic stroke and vascular
Ding Zhang1, Ni Jia2, Zhihan Hu3
1Guangxi university of chinese medicine Nanning, China.
Experimental Gerontology
|February 6, 2024
Summary
This study identified three key genes (GAS2L1, ARHGEF40, PFKFB3) as potential biomarkers for ischemic stroke and vascular dementia. These genes, linked to immune cell infiltration, show promise for early diagnosis and treatment strategies.
Area of Science:
- Neurology
- Immunology
- Genetics
Background:
- Ischemic stroke and vascular dementia are common cerebrovascular diseases with significant neurological and cognitive impacts.
- Current diagnostic tools are limited, and causative genes remain largely uninvestigated.
- Understanding the role of immune infiltration is crucial for identifying new biomarkers and therapeutic targets.
Purpose of the Study:
- To screen for novel biomarkers and analyze therapeutic targets for ischemic stroke and vascular dementia using an immune infiltration perspective.
- To identify key genes associated with these diseases and evaluate their diagnostic potential.
- To explore the relationship between identified genes and immune cell infiltration patterns.
Main Methods:
- Utilized gene expression profiles from the NCBI GEO database for ischemic stroke and vascular dementia.
- Employed LASSO regression and SVM-RFE algorithms to identify key genes.
- Conducted GO and KEGG enrichment analyses, CIBERSORT for immune cell quantification, and logistic regression for diagnostic modeling.
- Validated key genes using RT-PCR.
Main Results:
- Identified 105 intersecting differentially expressed genes (DEGs) with functions in metabolic processes and signaling pathways.
- Selected GAS2L1, ARHGEF40, and PFKFB3 as key diagnostic genes with high AUC values (0.882, 0.867, 0.869 respectively).
- Found significant correlations between these genes and immune cells like macrophage M1, B cells, and mast cells.
- RT-PCR confirmed significantly elevated mRNA levels of GAS2L1, ARHGEF40, and PFKFB3 in disease groups.
Conclusions:
- GAS2L1, ARHGEF40, and PFKFB3 are promising biomarkers for the early diagnosis of ischemic stroke and vascular dementia.
- Immune infiltration plays a significant role in the pathogenesis of these diseases.
- The developed immune infiltration-based models offer a new avenue for predicting diagnosis and guiding treatment.
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