Pipette Olympics: An Engaging Exercise for Undergraduate Laboratory Training.

Area of Science:

• Neuroscience
• Molecular Biology
• Biotechnology

Background:

• Pipetting is a fundamental laboratory technique crucial for molecular neuroscience and other biological sciences.
• The COVID-19 pandemic significantly limited undergraduate access to hands-on laboratory training.
• There is a need for standardized, accessible methods to re-teach essential lab skills.

Purpose of the Study:

• To develop and present a standardized, engaging protocol for teaching undergraduate students the fundamentals of pipetting and serial dilutions.
• To provide instructors with a competitive exercise to reinforce basic laboratory techniques.
• To offer a solution for reintroducing students to essential lab practices and standardizing training.

Main Methods:

• A standardized protocol was designed focusing on pipetting, serial dilutions, and basic statistical analysis.
• The protocol incorporates elements of friendly competition to enhance student engagement.
• Example results from an undergraduate and data from three students' initial attempts are provided.

Main Results:

• The protocol offers a structured approach to teaching and practicing fundamental laboratory skills.
• It provides a benchmark for assessing student proficiency in pipetting and dilution techniques.
• The exercise facilitates the standardization of training, saving valuable laboratory time.

Conclusions:

• This protocol provides an effective and engaging method for undergraduate neuroscience and biology students to relearn essential laboratory techniques.
• The standardized approach helps to mitigate learning gaps caused by pandemic-related disruptions.
• Implementing this protocol can enhance practical skills and confidence in early-career scientists.