Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A parametric study of mechanoporation through microfluidic design to modulate shear, compressive, and adhesion forces and loading rates.

Lab on a chip·2026

Same author

Mesoscale Modeling of Hydrogels Under Frictional Shear Stress.

Macromolecules·2026

Same author

Overcoming the Energy vs Power Dilemma in Commercial Li-Ion Batteries via Sparse Channel Engineering.

ACS energy letters·2024

Same author

Development of a microfluidic cell transfection device into gene-edited CAR T cell manufacturing workflow.

Lab on a chip·2023

Same author

Label-free microfluidic isolation of functional and viable lymphocytes from peripheral blood mononuclear cells.

Biomicrofluidics·2023

Same author

Effect of valve spacing on peristaltic pumping.

Bioinspiration & biomimetics·2023

Same journal

Rheology of <i>Escherichia coli</i> suspensions with various bacterial morphologies and motion characteristics.

Soft matter·2026

Same journal

Stress-boundary-memory feedback drives vortical-polar transitions in softly confined active matter.

Soft matter·2026

Same journal

CAGE ionic liquids meet biomembranes: unraveling molecular mechanisms and partitioning kinetics.

Soft matter·2026

Same journal

Steady and oscillatory propulsion in reactive swimming droplets.

Soft matter·2026

Same journal

Axial forces in capillary liquid bridges of polymer solutions.

Soft matter·2026

Same journal

Dual-mode pH-programmable enzymatic hydrogel system for on-demand glucose generation.

Soft matter·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 4, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Adhesion-based high-throughput label-free cell sorting using ridged microfluidic channels.

Fatima Ezahra Chrit¹, Peiru Li¹, Todd Sulchek¹

¹George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA. alexander.alexeev@me.gatech.edu.

|February 7, 2024

Summary

This summary is machine-generated.

This study introduces a novel microfluidic device for label-free cell sorting based on molecular surface marker affinity. The method utilizes differential cell trajectories influenced by adhesion strength and elasticity for high-throughput separation.

More Related Videos

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Related Experiment Videos

Last Updated: Jul 4, 2025

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

Published on: August 30, 2007

Area of Science:

Biotechnology
Cell Biology
Microfluidics

Background:

Accurate cell identification and sorting are crucial for medical diagnostics, cell engineering, and biotechnology.
Existing methods often require cell labeling, which can be costly and time-consuming.

Purpose of the Study:

To develop a high-throughput, label-free microfluidic method for sorting biological cells based on molecular surface marker affinity.
To investigate the influence of cell adhesion strength and elasticity on sorting efficiency.

Main Methods:

A microfluidic channel design featuring periodic skewed ridges and adhesive coatings was employed.
Three-dimensional computer simulations were used to analyze cell trajectories within the microchannels.
The effect of varying cell elasticity and microchannel ridge angles on sorting was examined.

Main Results:

Cell trajectories in the microchannels were found to be sensitive to cell adhesion strength, enabling adhesion-based separation.
Cell elasticity was identified as a factor that can enhance the resolution of cell sorting.
Microchannel ridge angle tuning was shown to improve sorting efficiency for cells with different mechanical properties.

Conclusions:

The developed microfluidic method offers a promising label-free approach for high-throughput cell sorting.
Leveraging differential cell adhesion and elasticity provides a robust mechanism for precise cell separation.
This technology has potential applications in various fields requiring specific cell population isolation.