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Isothermal amplification-based microfluidic devices for detecting foodborne pathogens: a review
Thi Ngoc Diep Trinh1, Nguyen Nhat Nam2
1Department of Materials Science, School of Applied Chemistry, Tra Vinh University, Tra Vinh City 87000, Vietnam. ttndiep@tvu.edu.vn.
Analytical Methods : Advancing Methods and Applications
|February 7, 2024
Summary
Isothermal amplification methods integrated into microfluidic devices offer a promising solution for on-site detection of foodborne pathogens, overcoming limitations of traditional polymerase chain reaction (PCR). This review highlights advances in these integrated systems.
Area of Science:
- Biotechnology and Biomedical Engineering
- Food Safety and Diagnostics
- Molecular Biology
Background:
- Polymerase chain reaction (PCR) is the standard for nucleic acid amplification but requires complex equipment, hindering microfluidic integration for on-site foodborne pathogen detection.
- Microfluidic platforms offer potential for convenient, cost-effective, and efficient foodborne pathogen control.
- Isothermal amplification methods are explored as alternatives to PCR for simplified microfluidic integration.
Purpose of the Study:
- To review advancements in isothermal amplification-based microfluidic devices for detecting foodborne pathogens.
- To discuss the integration of nucleic acid extraction methods within microfluidic platforms.
- To analyze microfluidic platforms utilizing loop-mediated isothermal amplification, recombinase polymerase amplification, and recombinase-aided amplification.
Main Methods:
- Review of existing literature on nucleic acid amplification techniques and microfluidic integration.
- Analysis of different nucleic acid extraction strategies suitable for microfluidic systems.
- Discussion of specific isothermal amplification methods (LAMP, RPA, RAA) integrated into microfluidic devices.
Main Results:
- Isothermal amplification methods are well-suited for microfluidic integration, enabling on-site detection without complex thermal cycling.
- Various nucleic acid extraction techniques can be effectively integrated into microfluidic platforms.
- Specific isothermal amplification methods like LAMP, RPA, and RAA have been successfully incorporated into microfluidic devices for pathogen detection.
Conclusions:
- Isothermal amplification-based microfluidic devices represent a significant advancement for rapid, on-site detection of foodborne pathogens.
- These integrated systems offer a viable alternative to traditional PCR, addressing limitations in portability and complexity.
- Further development in this area holds promise for improved food safety monitoring and public health.
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