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Live Organoid Cyclic Imaging
David E Reynolds1, Yusha Sun2, Xin Wang2
1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|February 7, 2024
Summary
Researchers developed a novel multiplexed cyclic imaging technique using bioorthogonal click chemistry to analyze patient-derived glioblastoma organoids. This method enables detailed molecular profiling while preserving organoid viability and spatial information for disease modeling.
Area of Science:
- Biomedical Engineering
- Molecular Imaging
- Cancer Biology
Background:
- Organoids are crucial for modeling human diseases due to their complexity.
- Existing imaging techniques like 4D live imaging and light-sheet imaging have limitations in cyclic and multiplexed analysis.
- Preserving spatial information is vital for comprehensive organoid profiling.
Purpose of the Study:
- To introduce a new bioorthogonal click chemistry-based imaging technique for organoids.
- To demonstrate multiplexed cyclic imaging for glioblastoma organoids.
- To enable accurate molecular profiling while maintaining organoid viability.
Main Methods:
- Application of bioorthogonal click chemistry for signal quenching.
- Development of a multiplexed cyclic imaging approach.
- Utilizing patient-derived glioblastoma organoids for validation.
Main Results:
- Successful demonstration of multiplexed cyclic imaging in live and fixed glioblastoma organoids.
- The technique allows for efficient molecular profiling of complex phenotypes.
- Glioblastoma markers were screened in patient-derived organoids with preserved viability.
Conclusions:
- Bioorthogonal click chemistry provides a versatile tool for multiplexed cyclic imaging of organoids.
- This technology enhances the molecular profiling of organoids, aiding disease modeling.
- The approach has broad potential for studying physiological developments in various organoid systems.
