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Related Experiment Video

Updated: Jul 4, 2025

Author Spotlight: In Vitro Investigations of Circadian Rhythms in Multicellular Systems

Author Spotlight: In Vitro Investigations of Circadian Rhythms in Multicellular Systems

Published on: February 16, 2024

Live Organoid Cyclic Imaging.

David E Reynolds¹, Yusha Sun², Xin Wang²

¹Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, 19104, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|February 7, 2024

Summary

This summary is machine-generated.

Researchers developed a novel multiplexed cyclic imaging technique using bioorthogonal click chemistry to analyze patient-derived glioblastoma organoids. This method enables detailed molecular profiling while preserving organoid viability and spatial information for disease modeling.

Keywords:

biomarker discovery bioorthogonal click‐chemistry glioblastoma longitudinal monitoring multiplexing organoids

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Area of Science:

Biomedical Engineering
Molecular Imaging
Cancer Biology

Background:

Organoids are crucial for modeling human diseases due to their complexity.
Existing imaging techniques like 4D live imaging and light-sheet imaging have limitations in cyclic and multiplexed analysis.
Preserving spatial information is vital for comprehensive organoid profiling.

Purpose of the Study:

To introduce a new bioorthogonal click chemistry-based imaging technique for organoids.
To demonstrate multiplexed cyclic imaging for glioblastoma organoids.
To enable accurate molecular profiling while maintaining organoid viability.

Main Methods:

Application of bioorthogonal click chemistry for signal quenching.
Development of a multiplexed cyclic imaging approach.
Utilizing patient-derived glioblastoma organoids for validation.

Main Results:

Successful demonstration of multiplexed cyclic imaging in live and fixed glioblastoma organoids.
The technique allows for efficient molecular profiling of complex phenotypes.
Glioblastoma markers were screened in patient-derived organoids with preserved viability.

Conclusions:

Bioorthogonal click chemistry provides a versatile tool for multiplexed cyclic imaging of organoids.
This technology enhances the molecular profiling of organoids, aiding disease modeling.
The approach has broad potential for studying physiological developments in various organoid systems.