Jove
Visualize
Contact Us
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Ecsit Facilitates Memory Cd8+ T Cell Development By Mediating Fumarate Synthesis During Viral Infection And Tumorigenesis.
  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Ecsit Facilitates Memory Cd8+ T Cell Development By Mediating Fumarate Synthesis During Viral Infection And Tumorigenesis.

Related Experiment Video

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

8.7K

ECSIT facilitates memory CD8+ T cell development by mediating fumarate synthesis during viral infection and tumorigenesis.

Yongbing Yang1,2, Yanan Wang1, Zhongcheng Wang3

  • 1Department of Immunology, State Key Laboratory of Reproductive Medicine, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Collaborative Innovation Center for Personalized Cancer Medicine, Gusu School, the Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, National Vaccine Innovation Platform, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China.

Nature Cell Biology
|February 7, 2024

View abstract on PubMed

Summary
This summary is machine-generated.

ECSIT is vital for developing memory CD8+ T cells by regulating fumarate synthesis and TCF-1 expression. This finding offers new therapeutic strategies for viral infections and cancer immunotherapy.

Area of Science:

  • Immunology
  • Cell Biology
  • Cancer Research

Background:

  • Memory CD8+ T cells are critical for adaptive immunity against infections and cancer.
  • Transcriptional programs of memory T cells are known, but regulatory mechanisms for their formation remain unclear.

Purpose of the Study:

  • To identify key regulatory mechanisms controlling CD8+ T cell memory formation.
  • To investigate the role of ECSIT in memory CD8+ T cell differentiation.

Main Methods:

  • Investigated the role of ECSIT in T cells using genetic ablation models.
  • Analyzed fumarate synthesis, TCF-1 expression, and its promoter methylation.
  • Correlated ECSIT expression with stem-like memory progenitor exhausted CD8+ T cells and patient survival.

Main Results:

  • ECSIT is essential for memory CD8+ T cell differentiation.
  • ECSIT deficiency leads to loss of fumarate synthesis and abrogated TCF-1 expression via KDM5-mediated demethylation.
  • ECSIT expression positively correlates with stem-like memory progenitor exhausted CD8+ T cells and cancer patient survival.

Conclusions:

  • ECSIT-mediated fumarate synthesis promotes TCF-1 activity and memory CD8+ T cell development in viral infections and cancer.
  • ECSIT is a crucial regulator of memory CD8+ T cell formation.
  • Therapeutic strategies involving fumarate analogues and PD-L1 inhibition show promise for tumor immunotherapy.

More Related Videos

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells
06:07

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

276
Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges
05:03

Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges

Published on: April 26, 2024

689

Related Experiment Videos

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development
09:03

Rapid In Vivo Assessment of Adjuvant's Cytotoxic T Lymphocytes Generation Capabilities for Vaccine Development

Published on: June 19, 2018

8.7K
Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells
06:07

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

276
Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges
05:03

Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges

Published on: April 26, 2024

689

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

735
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
735
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

917
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
917
T Cell Types and Functions01:24

T Cell Types and Functions

1.0K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.0K
Immunological Memory01:23

Immunological Memory

616
Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
616
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies