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Lactobacillus Persisters Formation and Resuscitation.

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Summary

This study reveals that Lactobacillus forms persister cells, dormant cells aiding survival, in response to antibiotics like ampicillin. These cells exhibit typical persister characteristics, offering insights into Lactobacillus survival mechanisms.

Keywords:
Lactobacilluspersistencepersister resuscitation

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Area of Science:

  • Microbiology
  • Bacterial Physiology

Background:

  • Lactobacillus is a key probiotic, but its stress response mechanisms, particularly persister cell formation, remain understudied compared to pathogenic bacteria.
  • Persister cells are dormant bacterial variants crucial for surviving environmental stresses, yet their role in Lactobacillus is largely unexplored.

Purpose of the Study:

  • To investigate the formation and characteristics of persister cells in Lactobacillus strains.
  • To understand the conditions necessary for Lactobacillus persister cell development and their resuscitation patterns.

Main Methods:

  • Treatment of Lactobacillus strains with beta-lactam antibiotics (ampicillin, amoxicillin).
  • Utilized kill curves and transmission electron microscopy to identify and characterize persister cells.
  • Employed single-cell analysis to observe the resuscitation dynamics of these dormant cells.

Main Results:

  • Lactobacillus forms a small subpopulation (0.0001-1%) of persister cells when exposed to beta-lactam antibiotics.
  • Approximately 0.5-1% of these persister cells heterogeneously resuscitated upon addition of fresh media.
  • Identified key persister cell traits in Lactobacillus, including dormancy, multi-drug tolerance, and typical resuscitation patterns.

Conclusions:

  • This research establishes a method for inducing and verifying persister cells in Lactobacillus.
  • Antibiotic-induced Lactobacillus persister cells share common characteristics with persister cells from other bacterial species.
  • The study suggests that bacterial species-specific traits, like membrane structure, may influence persister formation and resuscitation mechanisms.