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Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component, Mios
Miranda L Wilson1, Shannon N Romano1, Nitya Khatri1
1Department of Cell, Developmental, and Regenerative Biology. Icahn School of Medicine at Mount Sinai. One Gustave L. Levy Place Box 1020 New York, NY 10029-6574.
Biorxiv : the Preprint Server for Biology
|February 8, 2024
Summary
The RNA binding protein Rbpms2 acts as a crucial switch in zebrafish, directing early gonocytes towards ovary development by repressing male factors and promoting female factors.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- Proper biological sex establishment is vital for reproductive success.
- In many species, gonads initially develop as ovaries, requiring precise genetic regulation.
- The RNA binding protein Rbpms2 (RNAbp) has been previously identified as essential for ovary fate determination in zebrafish.
Approach:
- Identified Rbpms2 targets in oocytes, termed Rbpms2-bound oocyte RNAs (rboRNAs).
- Investigated Rbpms2's role as a translational regulator of these target RNAs.
- Utilized genetic analyses to explore Rbpms2's interaction with the mTorc1 signaling pathway and its component Mios (Missing oocyte).
Key Points:
- Rbpms2 regulates the translation of rboRNAs, including factors involved in testis development and ribosome biogenesis.
- Rbpms2 promotes nucleolar amplification through the mTorc1 signaling pathway, specifically via the Gator2 component Mios.
- Rbpms2 functions as a binary fate-switch in early bipotential gonocytes, repressing testis factors and promoting oocyte factors.
Conclusions:
- Rbpms2 integrates the regulation of sexual differentiation factors with nutritional availability pathways during zebrafish oogenesis.
- Rbpms2 ensures oocyte progression through a critical Gator2-mediated checkpoint, essential for ovary development.
- These findings reveal a novel mechanism controlling sexual fate decisions in early gonadal development.
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