Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Psychedelics relax predictive processing in the post-acute period by remodeling cortico-cortical feedback circuits.

bioRxiv : the preprint server for biology·2026
Same author

Different Retinoid Micellar Formulations on Wound Healing: Efficacy and Collagen Structure.

Pharmaceutics·2026
Same author

Neuronal microexons modulate arousal via the cAMP-PKA-CREB pathway in zebrafish.

Science advances·2026
Same author

Computational optimization of two-photon holographic stimulation sites<i>in vivo</i>.

Journal of neural engineering·2026
Same author

Visual information is broadcast among cortical areas in discrete channels.

eLife·2026
Same author

Open-source modular field-programmable gate array system for two-photon mesoscope enabling multiarea, multidepth neural activity recording and lifetime imaging.

Neurophotonics·2026
Same journal

A human-specific genetic modifier reconfigures large-scale cortical network dynamics underlying behavioral performance.

bioRxiv : the preprint server for biology·2026
Same journal

<i>Staphylococcus aureus</i> uses a eukaryotic-like uridyltransferase to make UDP-GlcNAc for cell wall synthesis.

bioRxiv : the preprint server for biology·2026
Same journal

Dynamic redistribution of eIF4F controls cap-dependent translation initiation.

bioRxiv : the preprint server for biology·2026
Same journal

When does additional information improve accuracy of RNA secondary structure prediction?

bioRxiv : the preprint server for biology·2026
Same journal

Normative brain-state trajectories reveal deviation from healthy aging in Alzheimer's disease.

bioRxiv : the preprint server for biology·2026
Same journal

Noradrenergic infraslow rhythm during sleep is the critical link between heart-rate dynamics and memory consolidation.

bioRxiv : the preprint server for biology·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2025

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K

Standardised Measurements for Monitoring and Comparing Multiphoton Microscope Systems.

Robert M Lees1, Isaac H Bianco2, Robert A A Campbell3

  • 1Science and Technology Facilities Council, Octopus imaging facility, Research Complex at Harwell, Harwell Campus, Oxfordshire, UK.

Biorxiv : the Preprint Server for Biology
|February 8, 2024
PubMed
Summary
This summary is machine-generated.

This protocol provides a standardized method for evaluating multiphoton microscopy hardware. It ensures scientists can verify their microscope

More Related Videos

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.6K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

938

Related Experiment Videos

Last Updated: Jul 4, 2025

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.9K
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

15.6K
Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy
08:43

Author Spotlight: Standardizing Spheroid Formation Methods for Metabolic and Oxygenation Analysis Using Fluorescence Lifetime Imaging Microscopy

Published on: August 9, 2024

938

Area of Science:

  • Optical microscopy
  • Scientific instrumentation

Background:

  • Multiphoton microscopy is a crucial technique in biological research, but hardware performance can vary.
  • Consistent and reliable data acquisition depends on well-characterized microscopy systems.

Approach:

  • This protocol focuses on standardizing the characterization of multiphoton microscopy hardware.
  • It provides a user-friendly method for assessing system performance and data consistency.
  • Software and data analysis are considered only as they directly relate to hardware performance.

Key Points:

  • Enables scientists, regardless of optical expertise, to evaluate their multiphoton microscope.
  • Facilitates the assessment of hardware performance and data reproducibility over time.
  • Aims to improve the reliability and comparability of multiphoton microscopy data.

Conclusions:

  • Implementing this protocol will lead to better characterization of multiphoton microscopy hardware.
  • It supports consistent data generation and enhances the overall utility of these advanced imaging systems.