Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectrometers01:25

IR Spectrometers

3.1K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Detection of Orthotopic Liver Cancer in Mice Using Indocyanine Green and Dynamic Diffuse Fluorescence Tomography Imaging.

Journal of biophotonics·2025
Same author

A clinical evaluation of amlexanox oral adhesive pellicles in the treatment of recurrent aphthous stomatitis and comparison with amlexanox oral tablets: a randomized, placebo controlled, blinded, multicenter clinical trial.

Trials·2009
Same author

Long-term assessment of bladder and bowel dysfunction after radical hysterectomy.

Gynecologic oncology·2009
Same author

Oxidative stress contributes to silica nanoparticle-induced cytotoxicity in human embryonic kidney cells.

Toxicology in vitro : an international journal published in association with BIBRA·2009
Same author

A rapid and simple method for identifying Mycobacterium tuberculosis W-Beijing strains based on detection of a unique mutation in Rv0927c by PCR-SSCP.

Microbes and infection·2009
Same author

CO oxidation over AuPd(100) from ultrahigh vacuum to near-atmospheric pressures: the critical role of contiguous Pd atoms.

Journal of the American Chemical Society·2009

Related Experiment Video

Updated: May 4, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

12.4K

IR780-based diffuse fluorescence tomography for cancer detection.

Zhuanxia He1, Limin Zhang1, Lingxiu Xing1

  • 1College of Precision Instrument and Optoelectronics Engineering, Tianjin University, Tianjin, China.

Journal of Biophotonics
|February 8, 2024
PubMed
Summary

This study used diffuse fluorescence tomography (DFT) to track IR780 iodide, a near-infrared agent, in tumors. DFT imaging revealed long-term IR780 retention in tumors and rapid liver metabolism, aiding cancer imaging development.

Keywords:
IR780diffuse fluorescence tomographypharmacokinetic parameterstumor detection

More Related Videos

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

12.2K
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

8.3K

Related Experiment Videos

Last Updated: May 4, 2026

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
12:24

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Published on: July 17, 2012

12.4K
Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery
11:05

Tissue-simulating Phantoms for Assessing Potential Near-infrared Fluorescence Imaging Applications in Breast Cancer Surgery

Published on: September 19, 2014

12.2K
Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation
07:05

Fluorescence-mediated Tomography for the Detection and Quantification of Macrophage-related Murine Intestinal Inflammation

Published on: December 15, 2017

8.3K

Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Pharmacokinetics

Background:

  • IR780 iodide is a near-infrared (NIR) contrast agent for cancer imaging and therapy.
  • Its accumulation, dynamics, and retention in biological tissues, particularly tumors, require further investigation.
  • Diffuse fluorescence tomography (DFT) offers a method for localizing and quantifying NIR fluorophores in 3D.

Purpose of the Study:

  • To comprehensively assess the biochemical and pharmacokinetic characteristics of IR780 using a homemade DFT system.
  • To evaluate the efficacy of IR780 for in vivo cancer imaging and pharmacokinetic analysis.
  • To determine optimal conditions and understand the biodistribution of IR780.

Main Methods:

  • Development and utilization of a homemade diffuse fluorescence tomography (DFT) imaging system.
  • Administration of IR780 iodide to mice bearing subcutaneous tumors.
  • In vivo and ex vivo imaging to assess tumor localization, biodistribution, and pharmacokinetics.
  • Mouse acute toxicity tests and cell assays to evaluate biocompatibility and cellular uptake.

Main Results:

  • An optimal IR780 concentration of 20 μg/mL was determined.
  • IR780 demonstrated good biocompatibility and significant cellular uptake.
  • DFT imaging successfully identified subcutaneous tumors and showed long-term retention of IR780 in tumors.
  • Rapid metabolism of IR780 was observed in the liver, with primary accumulation in tumors and lungs.
  • Sensitive tumor detection and pharmacokinetic rate analysis were achieved via DFT.

Conclusions:

  • DFT imaging provides valuable insights into the pharmacokinetics and biodistribution of IR780 in cancer models.
  • IR780 exhibits promising characteristics for tumor imaging due to its retention in tumors and detectable levels.
  • The findings support the further application and development of IR780 as a contrast agent in cancer diagnostics and therapeutics.