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Two at once: simultaneous increased production of astaxanthin and mycosporines in a single batch culture using a Phaffia rhodozyma mutant strain

  • 0Centro de Referencia en Levaduras y Tecnología Cervecera (CRELTEC), Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales (IPATEC), CONICET - Universidad Nacional del Comahue, Quintral 1250, San Carlos de Bariloche, Río Negro, 8400, Argentina. mmoline@comahue-conicet.gob.ar.
World Journal of Microbiology & Biotechnology +

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February 8, 2024

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February 8, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

A new mutant strain of Phaffia rhodozyma yeast overproduces astaxanthin and mycosporine-glutaminol-glucoside (MGG). This discovery paves the way for the commercial production of these valuable compounds for aquaculture, cosmetics, and sunscreens.

Area Of Science

  • Biotechnology
  • Microbial metabolite production
  • Yeast genetics

Background

  • Phaffia rhodozyma yeast produces astaxanthin (carotenoid) and mycosporine-glutaminol-glucoside (MGG, UV-B absorber).
  • Astaxanthin is valuable in aquaculture, cosmetics, and nutraceuticals; MGG has potential in natural sunscreens.
  • Industrial exploitation is limited by low yields, but simultaneous production of both metabolites could enhance value.

Purpose Of The Study

  • To develop a mutant strain of P. rhodozyma for overproduction of both astaxanthin and MGG.
  • To investigate the effects of carbon-to-nitrogen ratio and environmental factors on metabolite accumulation.
  • To characterize the accumulation patterns of astaxanthin and MGG during yeast growth.

Main Methods

  • Generation of a mutant strain of P. rhodozyma.
  • Quantification of astaxanthin and MGG production in the mutant and parental strains.
  • Analysis of metabolite accumulation under varying carbon-to-nitrogen ratios and different biotic/abiotic conditions (light, H2O2).

Main Results

  • The mutant strain exhibited significantly higher yields: 159% more astaxanthin (470.1 μg g⁻¹) and 220% more MGG (57.9 mg g⁻¹).
  • Astaxanthin accumulated during the exponential growth phase, while MGG accumulated during the stationary phase.
  • High C:N ratios favored astaxanthin, low ratios favored MGG; photosynthetically active radiation and low H2O2 stimulated both.

Conclusions

  • A novel hyper-producing mutant strain for both astaxanthin and MGG was successfully developed.
  • Understanding metabolite accumulation dynamics and influencing factors is crucial for optimizing simultaneous production.
  • This research represents a significant step towards the commercial exploitation of P. rhodozyma for dual metabolite manufacturing.

Keywords: