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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Related Experiment Video

Updated: Jul 4, 2025

Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy
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Measuring the Mechanical Properties of Living Cells Using Atomic Force Microscopy

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Protocol for measuring mechanical properties of live cells using atomic force microscopy.

Surya Bansi Singh1, Shatruhan Singh Rajput2, Shivprasad Patil3

  • 1National Centre for Cell Science, SP Pune University Campus, Pune 411007, India; SP Pune University, Pune 411007, India.

STAR Protocols
|February 8, 2024
PubMed
Summary

This study presents a protocol using Atomic Force Microscopy (AFM) to measure the viscoelastic properties of live cells. This method aids in understanding cell mechanics in both health and disease states.

Keywords:
AFMAtomic Force MicroscopyBiophysicsCell BiologyModel OrganismsPhysics

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Area of Science:

  • Biophysics
  • Cell Biology
  • Materials Science

Background:

  • Atomic Force Microscopy (AFM) is crucial for analyzing cellular physical properties.
  • Force-distance curves from AFM experiments reveal cell stiffness and viscoelasticity.

Purpose of the Study:

  • To present a detailed protocol for determining the viscoelasticity of live cells using AFM.
  • To enable the assessment of cell physical properties in various physiological and pathological conditions.

Main Methods:

  • Utilizing Atomic Force Microscopy (AFM) to acquire force-distance curves from live cells.
  • Applying the protocol to cell types including Drosophila hemocytes and mouse embryonic stem cells.

Main Results:

  • The protocol successfully determines the viscoelastic properties of live cells.
  • Demonstrated applicability to different cell types, indicating versatility.

Conclusions:

  • The presented AFM protocol offers a reliable method for quantifying live cell viscoelasticity.
  • This technique has significant potential for comparative studies of cell mechanics in healthy versus diseased states.