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Aptamer-based fluorescent sensor for highly sensitive detection of methamphetamine.

Yandan Wang1,2,3, Zheyu Wang1,2,3, Yishuo Tong1,2,3

  • 1School of Forensic Medicine, Shanxi Medical University, Jinzhong, Shanxi, P. R. China.

Luminescence : the Journal of Biological and Chemical Luminescence
|February 9, 2024
PubMed
Summary
This summary is machine-generated.

A new fluorescence aptasensor was developed for sensitive methamphetamine (METH) detection. This METH sensor utilizes graphene oxide and an aptamer, achieving a low detection limit in saliva and urine samples.

Keywords:
binding active sitefluorescent aptasensorgraphene oxide (GO)methamphetamine (METH)

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Nanotechnology

Background:

  • Methamphetamine (METH) is a dangerous illicit drug with significant public health implications.
  • Accurate and rapid detection of METH in biological samples is crucial for clinical and forensic applications.
  • Existing detection methods often face limitations in sensitivity, selectivity, or operational simplicity.

Purpose of the Study:

  • To develop a highly sensitive and selective fluorescence aptasensor for the detection of methamphetamine (METH).
  • To utilize graphene oxide (GO) as a platform for fluorescence quenching and signal restoration in the aptasensor.
  • To validate the aptasensor's performance in real biological samples like saliva and urine.

Main Methods:

  • A high-affinity aptamer (APT-40nt) was identified using molecular modeling and labeled with a fluorescein (FAM) dye.
  • The FAM-labeled aptamer was immobilized on a graphene oxide (GO) platform, leading to fluorescence quenching via π-stacking.
  • Methamphetamine (METH) detection was achieved by observing fluorescence restoration upon METH binding to the aptamer, causing its detachment from GO.

Main Results:

  • The fluorescence aptasensor demonstrated a linear detection range for METH from 1–50 and 50–200 nM.
  • An ultra-low detection limit of 0.78 nM was achieved under optimized conditions.
  • The aptasensor showed minimal interference from other illicit drugs and metabolites, with satisfactory recoveries (87%-111%) in spiked saliva and urine samples.

Conclusions:

  • The developed fluorescence aptasensor provides a sensitive, selective, and cost-effective method for METH detection.
  • The aptasensor is suitable for on-site detection of METH in biological fluids like saliva and urine.
  • This approach offers a promising strategy for rapid screening and monitoring of METH abuse.