"Cell Disk" DNA Storage System Capable of Random Reading and Rewriting

Area of Science:

• Biotechnology
• Synthetic Biology
• Information Storage

Background:

• DNA offers high storage density and durability, making it attractive for data storage.
• Current DNA storage systems struggle with simultaneous random reading and rewriting, limiting practical applications.

Purpose of the Study:

• To develop a DNA-based storage system enabling simultaneous random reading and rewriting.
• To enhance the practicability of DNA data storage for large-scale applications.

Main Methods:

• A
• Cell Disk
• system using yeast cells as storage units was designed.
• A customized CRISPR/Cas9
• lock-and-key
• module was integrated for selective data access and modification.
• A lossless compression codec algorithm was developed to increase information density per cell.

Main Results:

• Demonstrated target-specific reading and rewriting of compressed data in a mimic cell
• disk
• with up to 10⁵
• blocks
• .
• Achieved high specificity and reliability in data retrieval and modification.
• The system successfully supports concurrent random reading and rewriting.

Conclusions:

• The
• Cell Disk
• system overcomes limitations of current DNA storage by enabling simultaneous random read/write operations.
• This technology shows great scalability potential for practical, high-density in vivo DNA data storage.