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Solid-phase radioimmunoassay for relaxin.

J Gutkowska, J St-Louis, J Genest

    Clinical and Investigative Medicine. Medecine Clinique Et Experimentale
    |January 1, 1985
    PubMed
    Summary
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    A new radioimmunoassay (RIA) for porcine relaxin offers sensitive detection and allows for clinical evaluation of relaxin levels. While cross-reactive with rat and human samples, it shows high specificity for porcine relaxin.

    Area of Science:

    • Endocrinology
    • Immunochemistry
    • Assay Development

    Background:

    • Relaxin is a crucial hormone in reproduction.
    • Accurate measurement of relaxin is vital for clinical and research applications.
    • Existing methods may lack specificity or sensitivity for certain applications.

    Purpose of the Study:

    • To develop and characterize a specific radioimmunoassay (RIA) for porcine relaxin.
    • To assess the assay's specificity and cross-reactivity with other species' relaxin.
    • To enable clinical evaluation of plasma relaxin concentrations.

    Main Methods:

    • Development of antibodies in rabbits immunized with purified porcine relaxin.
    • Adsorption of antibodies onto polystyrene tubes.
    • Iodination of porcine relaxin using the Bolton-Hunter method for standards.

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  • Validation of sensitivity (31.3–8,000 pg/tube) and cross-reactivity studies.

    • Main Results:

    • The RIA demonstrated high sensitivity for porcine relaxin.
    • Rat relaxin showed cross-reactivity but required significantly higher concentrations for displacement.
    • Human and rat plasma showed displacement, but at much lower levels compared to porcine relaxin.
    • No cross-reactivity was observed with insulins from human, bovine, or porcine sources.

    Conclusions:

    • The developed RIA is specific for porcine relaxin, with limited cross-reactivity to rat and human relaxin.
    • This assay facilitates the clinical assessment of plasma relaxin during gestation and associated diseases.
    • Potential underestimation of human plasma relaxin concentrations may occur due to low cross-reactivity.