Optimization of total flavonoids purification process in rose by uniform design method

Area of Science:

• Natural Product Chemistry
• Adsorption Science

Background:

• Roses are a rich source of bioactive compounds, including flavonoids.
• Efficient extraction and purification methods are crucial for utilizing the potential of rose flavonoids.

Purpose of the Study:

• To establish an effective method for purifying total flavonoids from roses using macroporous resin chromatography.
• To optimize the purification process for maximizing recovery and purity.

Main Methods:

• Screening of six macroporous resins to identify the optimal adsorbent (X5 resin).
• Adsorption behavior analysis using kinetic and isotherm models.
• Optimization of purification parameters using uniform design methodology.
• Evaluation of antioxidant properties of purified total flavonoids.

Main Results:

• X5 macroporous resin demonstrated superior adsorption and desorption capacity for rose flavonoids.
• Optimized conditions (2 mg/mL sample conc., pH 2, 75% ethanol eluent) yielded 57.82% total flavonoid content.
• Achieved an 84.93% recovery rate for total flavonoids.
• Purified flavonoids exhibited substantial antioxidant potential.

Conclusions:

• The developed method using X5 macroporous resin is a dependable approach for purifying total flavonoids from roses.
• The purified rose flavonoids possess significant antioxidant properties, warranting further investigation and application.