New Highly Sensitive and Specific Raman Probe for Live Cell Imaging of Mitochondrial Function

Area of Science:

• Chemical Biology
• Cellular Imaging
• Spectroscopy

Background:

• Raman hyperspectral imaging offers sensitive subcellular visualization.
• Novel probes are needed for specific detection in the biological silent region (1800-2800 cm⁻¹).
• Mitochondrial function is critical for cell survival and requires reliable monitoring.

Purpose of the Study:

• To develop a novel Raman probe for live-cell imaging.
• To enable selective visualization and tracking of mitochondrial biochemical changes.
• To support Raman imaging of cellular processes in an in vitro model.

Main Methods:

• Synthesis of a novel Raman probe (RAR-BR).
• Application of the probe for Raman hyperspectral detection in live endothelial cells.
• Utilizing the biological silent region for enhanced signal detection.

Main Results:

• The RAR-BR probe demonstrated high sensitivity and selectivity for mitochondria.
• Successful imaging of mitochondria in live endothelial cells was achieved.
• The probe facilitated tracking of biochemical changes associated with mitochondrial function.

Conclusions:

• The developed RAR-BR probe is a valuable tool for live-cell Raman imaging.
• This technology allows for sensitive and selective monitoring of mitochondrial function.
• The probe supports the study of cellular processes at the subcellular level.