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Updated: Jul 19, 2026

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
Published on: January 28, 2015
Protocol for neurogenin-2-mediated induction of human stem cell-derived neural progenitor cells
Ellen J Guss1, Laila Sathe2, Alexander Dai2
1Department of Stem Cell and Regenerative Biology, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA 02138, USA.
Abstract:
Human pluripotent stem cell-derived neural progenitor cells (NPCs) are an essential tool for the study of brain development and developmental disorders such as autism. Here, we present a protocol to generate NPCs rapidly and reproducibly from human stem cells using dual-SMAD inhibition coupled with a brief pulse of mouse neurogenin-2 (Ngn2) overexpression. We detail the 48-h induction scheme deployed to produce these cells-termed stem cell-derived Ngn2-accelerated progenitor cells-followed by steps for expansion, purification, banking, and quality assessment. For complete details on the use and execution of this protocol, please refer to Wells et al.1.
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