Guanosine-Based Multidrug Strategy Delivery for Synergistic Anti-Inflammation

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Natural Product Chemistry

Background:

• Combination therapy using natural products requires effective codelivery systems.
• Targeted delivery of multiple drugs via a single carrier presents significant challenges.
• Inflammatory bowel disease (IBD) necessitates innovative therapeutic strategies.

Purpose of the Study:

• To design and prepare a multi-drug-loaded hydrogel for synergistic codelivery of quercetin, demethyleneberberine, and dencichine.
• To investigate the responsive release mechanisms and physicochemical properties of the hydrogel carrier.
• To evaluate the efficacy of the codelivery system in suppressing inflammatory responses relevant to IBD.

Main Methods:

• Fabrication of a G4-quadruplex-based hydrogel incorporating three natural products.
• Utilized borate ester linkages for responsive release of catechol drugs and hydrogel degradation for dencichine release.
• Characterization using NMR, CD, and TEM; in vitro degradation and biocompatibility assays.
• In vitro assessment of inflammatory cytokine (TNF-α, IL-6) suppression.

Main Results:

• Successfully prepared and characterized a multi-drug-loaded hydrogel with controlled release capabilities.
• Demonstrated good biocompatibility and safety of the hydrogel, suggesting potential for injectable administration.
• Significant suppression of key inflammatory cytokines (TNF-α, IL-6) was observed.
• The system leverages synergistic effects of the natural products for enhanced therapeutic outcomes.

Conclusions:

• A simple and effective strategy for constructing a multi-drug-loaded hydrogel carrier was developed.
• The codelivery system facilitates synergistic therapy for natural products, offering a promising approach for IBD treatment.
• This work advances the field of natural product combination therapy through innovative drug delivery.